Versuch einer Kausalanalyse der geotropischen Reaktionskette im Chara-Rhizoid

Summary In the initial phase of the geotropical reaction of the Chara rhizoid the growth difference postulated by Sievers (1967c) between the physically upper, slightly subapical flank and the lower one is demonstrated. In horizontal exposure the growth of the extreme cell apex is continued, while the growth of the lower flank is inhibited and that of the upper one is promoted. In the end phase the cell apex shows a damped oscillation until it finally reaches the vertical growth direction. The statoliths follow the oscillating growth of the cell tip from one flank to the opposite one until they are statistically equally redistributed in their normal position.—In vertical exposure under reduced turgor pressure the statoliths fall down into the extreme cell apex, where they inhibit the growth of this part of the cell wall, while the subapical wall grows transversally.—It is concluded that the statoliths inhibit the growth of the cell wall area which they cover.—The physical phase of the reaction chain, the susception, is the gravity-induced downward displacement of the statoliths. The physiological phase starts with the diversion of the acropetal transport of the Golgi vesicles to the upper part of the cell, which is caused by the block of statoliths (perception). The greater rate of vesicle incorporation into the upper flank in comparison to the lower one causes the subapical growth difference which results in the curvature (reaction).—In the case of the Chara rhizoid Golgi- and statolith-apparatus function as a self-regulating cellular system.

---

---

Herrn Prof. Dr. Dr. h. c. Kurt Mothes zum 70. Geburtstag.     

Studies of the ADP/ATP carrier of mitochondria with fluorescent ADP analogue formycin diphosphate

The ADP/ATP carrier was studied by a fluorescent substrate, formycin diphosphate which is the only fluorescent ADP analogue to bind. Its low quantum yield, short decay time and spectral overlap with tryptophan has as yet prevented its wider use.By incorporating fluorescent acceptors of formycin diphosphate fluorescence, anthracene-maleimide and vinylanthracene, into the membrane, these difficulties were circumvented. Only bound formycin diphosphate transfers energy to the probes so that the secondary emission of these probes is a measure for membrane-bound formycin diphosphate.The fluorescent transfer is inhibited by ADP, bongkrekate and carboxy-atractylate whether added before or after incubation of formycin diphosphate showing that only binding to the adenine nucleotide carrier is measured. It also shows directly that the earlier demonstrated ADP fixation by bongkrekate is indeed a displacement into the matrix.The fluorescence decay time of the bound formycin diphosphate is measured as 1.95 ns compared to 0.95 ns of the free formycin diphosphate, indicating that formycin diphosphate is bound at the carrier in a non-polar environment.The depolarization decay time was found to be larger than 15 ns, indicating that carrier-bound formycin diphosphate is immobile within this time period.     

Über den Nucleolus der Nervenzelle

Zusammenfassung Bei supravitaler Fixierung und Anwendung besonderer Färbetechnik lassen sich in zahlreichen Nucleoli der Nervenzelle bei einer Reihe von Säugetieren vielfältige Strukturen erkennen, welche auch mit der Phasenkontrastoptik nachweisbar sind. Es läßt sich nachweisen, daß aus diesen Strukturen, die als Innenkörperchen bezeichnet werden. die Vakuolen entstehen, welche auf Grund des morphologischen Substrates als ein physiologisches Produkt des Nucleolus anzusehen sind. Damit erfahren die Vorstellungen von Körner (1937), C. und O. Vogt 1946, 1947) u. a. eine weitere Stütze.Die Untersuchungen wurden durch die Unterstützung der Deutschen Forschungsgemeinschaft ermöglicht.